FIG. 4.

HIRA and core histone H2B coimmunoprecipitate from cellular extracts. Material was precipitated from 500 μg of a K562 protein extract by using an irrelevant rabbit antiserum (lane 1), 1455 (lane 2), an irrelevant mouse monoclonal antibody (lane 3), or LG11.1 (lane 4), subjected to electrophoresis in a 15% acrylamide gel, and transferred for Western blotting analysis using the anti-HIRA antiserum 1455 (top) or the anti-H2B mouse antibody LG11.1 (bottom) as the staining reagent. Lanes T were loaded with 50 μg (10% input) of total extract. Note that each of the right-hand panels corresponds to a single exposure from the same blot.