FIG. 5.

Core histone-binding domains in HIRA protein. (A) HIRA deletion mutants indicated below the gels were radioactively labeled in vitro and tested for binding to GST, GST-H2B, and GST-H4 proteins immobilized on glutathione beads. (B and C) Binding of in vitro-translated H2B (B) and H4 (C) to HIRA deletion mutants. (D) The entire HIRA protein is schematized (top), with closed rectangles representing the seven WD repeats. HIRA deletion fragments are shown below with summarized H2B and H4 binding results. Numbers refer to amino acid residues in the HIRA protein sequence.