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. 2023 Sep 26;174(6):549–559. doi: 10.1093/jb/mvad069

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© The Author(s) 2023. Published by Oxford University Press on behalf of the Japanese Biochemical Society.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2 — DNA end flexibility of the H3.8 nucleosome. (A) Schematic diagram of the micrococcal nuclease (MNase) treatment assay. The DNA ends of the nucleosome are preferentially digested by MNase. After deproteinization, the resulting DNA fragments were analyzed by non-denaturing PAGE. (B) A representative gel image of the MNase treatment assay. The nucleosomes containing H3.3 (lanes 2–7) or H3.8 (lanes 8–13) were incubated in the presence of MNase for 0, 3, 6, 9, 12 and 15 min. The resulting DNA fragments were analyzed by non-denaturing PAGE with EtBr staining. The results were confirmed to be reproducible by two additional independent experiments (shown in Supplementary Fig. S1).