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Sir4p requires Sir3p to bind an internal tract of C1-3A/TG1-3 DNA. The indicated fusion proteins were expressed in HIS-Int-CA sir3 strains. Cells were spotted in 10-fold serial dilutions onto test plates (left) or control plates (right). The 3-AT concentration was 35 (A) or 50 (B) mM. Control plates were incubated for 4 days, and test plates were incubated for 17 (A) or 13 (B) days.
