Figure 3.
scRNA-seq analysis with tip or stalk cell enriched gene sets in VEGF treated 2D-cultured endothelium
(A) UMAP plots colored by ten differential transcriptomic subpopulations.
(B) UMAP plots indicate the heterogeneities of HUVECs with VEGF for 18h. Positive (blue) and negative (gray) of proliferation marks, ki-67. Values are shown in left bar relative to gray.
(C) Violin plots of ki-67 gene expression levels among the subgroups of 1–10. VEGF for 0 h (in red) and 18 h (in green).
(D) Dot plots of pseudo-bulk samples in the scRNA-seq in 0 h vs. 18 h VEGF-treated HUVEC cells. Tip and stalk enriched gene sets were shown in left and right, respectively.
(E and F) Violin plots of expression value for representative tip (E) and stalk (F) genes in 10 differential subpopulations (red, 0h; green, 18h VEGF treatment).