Figure 4.
Genome-wide FOXO1-ChIP-seqs analysis in VEGF-treated endothelium
(A) Correlation mapping with spearman score of 1st and 2nd FOXO1-ChIP-seqs.
(B) Pie-chart of FOXO1-binding genome region in HUVECs using cis-regulatory element annotation system.
(C) ChIP-qPCR validation of ESM1 loci from VEGF-treated HUVECs normalized to the input using the FOXO1-specific antibody and the control IgG.
(D) Venn diagram with overlapping si-FOXO1- or Ad-FOXO1-mediated up or downregulated genes and the genes involved in the enrichment regions from FOXO1-ChIP-seq. Each number indicates the gene volume. Purple indicates the common responsive gene number.
(E) FOXO1-ChIP-seq enrichment values are quantified with each tip vs. stalk mark categorized gene loci derived from the ref.32 N.D.: not detected.
(F) ANGPT2 and ESM1 integrated genome view with ChIP-seq enrichment of H3K4me3, H3K27Ac, and two independent FOXO1. Input indicates the negative control for FOXO1-ChIP-seq. MACS_peak bar indicates the significantly enriched region according to the MACS calculation methods.