Figure 5.

FOXO1 binds to both closed and open chromatin regions as a pioneer factor in the VEGF treated endothelium

(A) FOXO1 enrichment patterns are compared with those of H3K27me3, H3K4me3, and H3K27ac histone codes. The FOXO1 enrichment around the peaks of each histone code (±5 kb) in HUVECs.

(B) Integrated genome view of HOXA11 with 13 genome regions. Histone code information of H3K27ac (blue), H3K4me3 (black), and H3K27me3 (purple) from cells treated with VEGF for 0 h and 1 h, with adjusted peak levels shown at the upper left. Input indicates the negative control for FOXO1-ChIP-seq.

(C) Integrated genome view of the indicated tip cell enriched gene loci. Blue and red broken rectangles indicate FOXO1 bound at the H3K27me3 and H3K27ac enriched areas, respectively. FOXO1 enrichment on the CXCR4 genome locus was derived from single ChIP-seq data because of the failure to get profound FOXO1 enrichment on the locus, using MACS peak call with another ChIP-seq.

See also Figure S7.