Figure 3.

Surgical procedure

(A) Pup is anaesthetized. Operator holds pup in one hand and capillary in other. (Step 13).

(B and C) Pup is injected. Graphical representation in C shows area of injection marked by X. (Step 15–16).

(D‒F) Successful injection shows crescent of filled ventricle visualized using fast green. (Step 16).

(G and H) Electroporation. (Steps 17–18).

(I) Schematic coronal section indicating the expected location of targeted cells along the SVZ after successful injection and electroporation.

(J) Pups are returned to home cage away from the nest. Parent will collect and return the pup to the nest. (Step 21). See also Methods video S1.