REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
One Shot Stbl3 chemically competent E. coli (alternatives available)	Thermo Fisher Scientific	Cat#C737303
Chemicals, peptides, and recombinant proteins
Fast green (alternatives available)	Sigma	Cat#F7252
DNase-free water (alternatives available)	Invitrogen	Cat#AM9937
Dulbecco’s phosphate-buffered saline (alternatives available)	Sigma	D8537
IsoFlo	Apiece	5965901
Critical commercial assays
ZymoPURE II Plasmid Maxiprep Kit (alternatives available)	Zymo Research	Cat#D4203
InFusion cloning kit (alternatives available)	Clontech	Cat#638917
Experimental models: Organisms/strains
Mouse: C57BL/6J (alternatives available)	Charles River	RRID:IMSR_JAX:000664
Mouse: FVB/N (alternatives available)	Charles River	RRID:MGI:2165215
Recombinant DNA
Plasmid: piggyBase hGFAPmin-SpCas9-T2A-PBase	Garcia-Diaz et al.1	N/A
Plasmid: piggyBac CAG-EGFRviii_U6-sgCdkn2a_EF1a-tdTomato	Garcia-Diaz et al.1	N/A
Plasmid: piggyBac U6-sgNf1_U6-sgPten_U6-sgTrp53_EF1a-tdTomato	Garcia-Diaz et al.1	N/A
Plasmid: piggyBac U6-sgTrp53_EF1a-tdTomato_CAG-PdgfraD842V	Garcia-Diaz et al.1	N/A
Software and algorithms
SnapGene	N/A	RRID:SCR_015052
Other
NanoDrop (alternatives available)	Thermo Fisher Scientific	Cat# ND-ONE-W
Micropipette puller (e.g., Sutter Instrument Model P-97) (alternatives available)	Sutter Instrument	Model#P-97
Borosilicate glass capillaries (1.0 mm OD × 0.58 mm ID)	Harvard Apparatus	Cat#30-0019
15 cm dishes (alternatives available)	Starlab	Cat#CC7672-3614
Microloader pipette tips	Eppendorf	Cat#5242956003
0.22 μm PVDF sterile syringe filter (alternatives available)	Starlab	Cat#E4780-1221
35 mm dishes (alternatives available)	Corning	Cat#430165
Isofluorane anaesthetic delivery system – small animal system	VetTech Solutions	Cat#AN071
2 mL syringe (alternatives available)	Appleton Woods	Cat#BD574
Lamp with swan arm (alternatives available)	Intralux	Cat#4000-1
FemtoJet 4i microinjector	Eppendorf	Cat# 5252000021
Electrode gel (alternatives available)	Compex	Cat#602047
BTX Gemini X2 electroporator	BTX	Cat#45-2006
Tweezertrodes attachment for electroporator	BTX	Cat# 45-0488
Surgical skin marking pen (alternatives available)	Schuco	Cat#Z-SS-665