Table 2.

The primers incorporating adaptors and the two sides of targeting sequences in the 16s gene for microbiome profiling using the next generation sequencing platform.

Primer	Adaptor	16s gene targeting sequence
Forward	5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-3′	5′-CCTACGGGNGGCWGCAG-3′
Reverse	5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-3′	5′-GACTACHVGGGTATCTAATCC-3′