Table 1.

List of iM forming sequences used in this study

		iM / hybrid-ds/iM
iM	Sequence (5‘- > 3‘)	${\mathrm{T}}_{\mathrm{m}}^{\mathrm{in}-\mathrm{vitro}}$ (pH 7)	${\mathrm{pH}}_{\mathrm{T}}^{\mathrm{in}-\mathrm{vitro}}$ (20 °C)
hTel	d(C3TAA)3C3	n.d./ n.d.	6.20 ± 0.02/ 6.09 ± 0.01
hT121-6	d(C6T)3C6	24.9 ± 0.5/ 28.0 ± 0.4	6.94 ± 0.03/ 6.81 ± 0.02
hBcl-2	d(CAGC4GCTC3GC5T2C2TC3GCGC3GC4T)	12.9 ± 0.5/ -	6.48 ± 0.03 -
hPDGFa	d(C2GCGC4TC5GC5GC5GC13)	28.8 ± 1.2/ 27.6 ± 0.9	6.96 ± 0.03/ 6.96 ± 0.05
hRAD17	d(GCT2CTAGTCA2TC2AC9GC9G2A)	33.8 ± 0.5/ 34.9 ± 0.6	7.08 ± 0.02/ 7.10 ± 0.03

In the hybrid-ds/iM constructs, the ds segment d(GCTTCTAGTCAAT^*).d(TTGACTAGAAGC) was attached to the 5′-end of iM. T^* corresponds to the unpaired spacer nucleotide between ds and iM segments. For the complete list of sequences and modifications, see Supplementary Table 1. ${\mathrm{T}}_{\mathrm{m}}^{\mathrm{in}-\mathrm{vitro}}$ and ${\mathrm{pH}}_{\mathrm{T}}^{\mathrm{in}-\mathrm{vitro}}$ corresponds to melting temperature and transitional pH measured in-vitro (25 mM KPO_i, 10.5 mM NaCl, 110 mM KCl, 1 mM MgCl₂, 130 nM CaCl₂) at pH = 7 and T = 20 °C, respectively. The ${\mathrm{T}}_{\mathrm{m}}^{\mathrm{in}-\mathrm{vitro}}$ and ${\mathrm{pH}}_{\mathrm{T}}^{\mathrm{in}-\mathrm{vitro}}$ values for iM and corresponding hybrid-ds/iM construct are typeset on top and bottom, respectively. Error bars for the ${\mathrm{pH}}_{\mathrm{T}}^{\mathrm{in}-\mathrm{vitro}}$ values represent the 95% confidence interval of the calculated fit. Errors reported for ${\mathrm{T}}_{\mathrm{m}}^{\mathrm{in}-\mathrm{vitro}}$ values correspond to the standard error of the mean for each triplicate experiment. The experimental data are displayed in Supplementary Figs. 1 and 2.