Fig. 4.

a Quenching efficiency of NS@DA at different NS@DA content (FAM-ssDNA: 200 nM; NS@DA: 2–18 µL; quenching time: 30 min; temperature: 18 °C), b quenching efficiency of NS@DA at 4 °C, 18 °C and 30 °C (FAM-ssDNA: 200 nM; NS@DA: 10 µL; quenching time: 30 min.), c kinetic of Quenching efficiency of NS@DA (FAM-ssDNA: 200 nM; NS@DA: 10 µL; quenching time: 30 min, temperature: 18 °C), d Kinetic of recovery efficiency of NS@DA (FAM-ssDNA: 200 nM; NS@DA: 10 µL; quenching time: 30 min, temperature: 18 °C.). Total volume of measurement is 200 µL and in buffer (10 mM HEPES, pH 7.4, 2 mM CaCl₂)