TABLE 2.
Distribution of J in DNA studied by immunoprecipitation of J-containing DNA fragments
| Clone | HOMedU concn (mM) | % Efficiency of anti-J immunoprecipitationa in:
|
|||||
|---|---|---|---|---|---|---|---|
| Telomeric repeats | 50-bp repeats | Tubulin genes | VO2 VSG gene | 221 VSG gene | HYG gene | ||
| PF WT | 0 | 0 | 1 | 0 | 0 | 0 | |
| 1 | 7 | 11 | 7 | 28 | 32 | ||
| PF TKN | 1 | 9 | 14 | 2 | 28 | 35 | |
| BF 3174b | 0 | 31 | 12 | 0 | 8 | 2 | 0 |
| 1 | 19 | 17 | 30 | 52 | 8 | 9 | |
| 10 | 31 | 24 | 45 | 71 | 13 | 22 | |
| BF HTK | 0 | 31 | 12 | 1 | 6 | 0 | 0 |
| 1 | 15 | 15 | 28 | 39 | 16 | 21 | |
a
The efficiency of immunoprecipitation is expressed as the percentage of the total input that was bound by the antibody. Dot blots with immunoprecipitated DNA were hybridized with the probes indicated above each column. The 1% immunoprecipitation of 50-bp repeats in PF WT trypanosomes, the 1% immunoprecipitation of tubulin genes in BF HTK cells, and the 2% immunoprecipitation of the VSG 221 gene in BF 3174 cells were not reproducible and represent nonspecific (co)immunoprecipitation.
b
BF 3174 cells contain a HYG and a NEO gene in the active 221a VSG gene expression site.