TABLE 3.
Quantitation of the level of BrdU in DNA
| Clone | BrdU concn (μM) | n | Mol% ofa:
|
|
|---|---|---|---|---|
| BrdUa | dJb | |||
| PF WT | 0 | 1 | 0.0 | 0.0 |
| 1,000 | 1 | 12.9 | 0.0 | |
| PF TKN | 100 | 1 | 9.0 | 0.0 |
| BF WT | 0 | 2 | 0.0 | 0.14 |
| 100 | 3 | 5.2 | 0.05 | |
| 250 | 2 | 7.7 | 0.01 | |
| BF HTK | 0 | 2 | 0.0 | 0.12 |
| 50 | 1 | 7.7 | 0.02 | |
| 100 | 3 | 9.3 | 0.01 | |
a
The moles percent of BrdU in DNA and the level of dJ in control cells were determined by 32P postlabeling.
b
The recovery of J by postlabeling is incomplete and variable (see Materials and Methods); the presented values of dJ have been corrected for partial recovery. The relative decrease in the level of dJ caused by the incorporation of BrdU into DNA was quantitated by anti-J immunoblot analysis.