FIGURE 5.

FMR1 reactivation normalizes molecular abnormalities of FXS neurons. qRT- PCR analysis monitoring expression of REST (A) or DCC, ROBO3 and SLIT1 (B) in FXS 848- neurons expressing an FMR1-SF shRNA or treated with a small molecule FMR1-SF inhibitor. The expression of FMR1 in FXS 848-neurons is shown relative to that in normal neurons, which was set to 1. (C) Immunoblot analysis showing DGKK levels in FXS 848-neurons expressing an FMR1-SF shRNA or treated with a small molecule FMR1-SF inhibitor. DGKK levels in normal neurons are shown. The DGKK signal was quantified and normalized to that obtained in normal neurons, which was set to 100%. Data are represented as mean ± SD (n = 3 biological replicates). *P < 0.05, **P < 0.01.