Figure 4.

RNA-Seq experimental design and high-level functions affected by different regions of EF amplitude exposure for two glioma stem cell lines, GSC827 and GSC923. A. Treatment schema and dish arrangement. The treated dish is divided into three discrete regions: TC, TM, and TS. The average EF amplitude in each region is shown to the right. B. Unsupervised principal component analysis (PCA) of all samples for GSC827 and GSC923 while pooling treated (TC, TM, TS) and control regions (CC, CM, CS). Ellipses highlight the separation between cell lines. C, D. Unsupervised PCA of GSC827 and GSC923 separated by the treated dish region. E. Functional summary of the gene set enrichment analysis for the TS vs. TC, TS vs. TM, and TM vs. TC contrasts in both cell lines.