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. 2024 Feb 29;10(5):e27325. doi: 10.1016/j.heliyon.2024.e27325

Table 2.

Serological markers and other non-invasive tests for NAFLD-Related Fibrosis. Data from cross-sectional studies.

markers N
Training set/Validation set
AUC
Diagnostic accuracy
Training set Validation set
3D-MRE [66] 100 NR 0.981 at 60 Hz: AUC = 0.927
Cut-off = 3.40 kPa, PPV = 72.2%, NPV = 97.5% at 40 Hz: AUC = 0.981
Cut-off = 2.43 kPa, PPV = 72.2 %, NPV = 1.0%
2D-MRE [66] 100 NR 0.921 at 60 Hz: Cut-off = 3.80 kPa, PPV = 81.2%, NPV = 97.6%
Fibro MRI [69] 39/87 0.940 0.850 Training set: cut-off = 0.5, PPV = 77%, NPV = 86%
Validation set: cut-off = 0.5, PPV = 67%, NPV = 87%.
MRE [64] 104 NR 0.820 F2: AUC = 0.89, F3: AUC = 0.87, F4: AUC = 0.87
RP11‐128N14.5 lncRNA [83] 88/50 0.706 0.694 Training set: sensitivity = 73.7%, specificity = 70.4%
Validation set: sensitivity = 78.3%, specificity = 63%,
BNIP3L mRNAs [83] 88/50 0.676 0.686 Training set: sensitivity = 57.9%, specificity = 81.5%
Validation set: sensitivity = 51.9%, specificity = 91.3%,
Complement component C7 [88] 19 1 NR NR
α-2-macroglobulin [88] 19 0.870 NR NR
Complement component C8 γ chain [88] 19 0.800 NR NR
Fibulin-1 [88] 19 0.790 NR NR
α-1-antichymotrypsin [88] 19 0.800 NR NR
the FM-fibro LSM index [60] 249 0.943 0.941 cut-off = 7.201, PPV = 73.5%, NPV = 94.6%
top 10 metabolite panel [78] 156/59 0.940 0.840 Sensitivity = 90%, specificity = 79%
IGF-1 + ferritin + INR [82] 225 NR 0.810 PPV = 98%, NPV = 71%
TGFB2/TGFB2‐OT1
Test [83]
88/50 0.797 0.786 Training set: sensitivity = 65%, specificity = 81.3%
Validation set: sensitivity = 62.5%, specificity = 94.4%
TGFB2/TGFB2‐OT1 + FIB‐4 [83] 88/50 0.891 0.889 Training set: sensitivity = 80%, specificity = 87.5%
Validation set: sensitivity = 87.5%, specificity = 83.3%
7 eicosanoids panel [35] 426 0.740 NR NR

3D-MRE: Three-dimensional MRE; 2D-MRE: two-dimensional MRE; Fibro MRI: the diagnostic accuracy of significant fibrosis prediction; MRE; magnetic resonance elastography; the FM-fibro LSM index: combination of the FM-fibro index and LSM; top 10 metabolite panel: the top 10 serum metabolites including 5alpha-androstan-3beta monosulfate, pregnanediol-3-glucuronide, androsterone sulfate, epiandrosterone sulfate, palmitoleate, dehydroisoandrosterone sulfate, 5alpha-androstan-3beta disulfate, glycocholate, taurine and fucose; 7 eicosanoids panel: 5-HETE, 7,17-DHDPA, adrenic acid, arachidonic acid, eicosapentaenoic acid, 16-HDOHE, and 9-HODE.