Fig. 3.

EGCG down-regulated the expression of proinflammatory cytokine induced by LPS.

H9c2 were pretreated with EGCG (25 μM) for 1 h, then stimulated with LPS (1 μg/ml) for 12 h (A, B, C, D, E and F) Western blotting analysis was carried out to evaluate the levels of IL-6, TNF-α and IL-1β, n = 3 (Supplementary Fig. 2). (G, H and I) RT-qPCR analysis was carried out to evaluate the mRNA expression of IL-6, TNF-α and IL-1β, n = 6. Data represent the mean ± S.D. of three independent experiments. Statistical analysis was performed with one-way ANOVA followed by Bonferroni's correction. **P < 0.01, ***P < 0.001, #P < 0.0001.