FIG. 1.

Functional deletion mapping of a 2.6 kb DNA fragment of clone D3. (A) Restriction map of the genomic insert of clone D3. The arrow indicates the YHM2 ORF. The two putative TATA boxes (−54 and −80) and the putative HAP2-HAP3-HAP4 transcription factor-binding site (−559) are indicated. (B) Deletion analysis of the D3 sequence. To identify the gene of interest, the fragment between the two NcoI sites of YHM2 was deleted. The plasmid with the deletion (pY-D3Δ935–1085) was tested for its ability to complement the temperature-sensitive phenotype of abf2 mutant cells. (C) Clone D3 can suppress the temperature-sensitive defect of the abf2 mutant but not the deleted clone D3Δ935–1085. The abf2 mutant cells transformed with YEp13, pY-D3, and pY-D3Δ935–1085 were grown to late log phase in synthetic medium (−Ura, −Leu) containing glucose and were streaked onto YPG plates (3% glycerol as nonfermentable carbon source) and grown at 30 or 37°C.