FIG. 2.

The KLR17-3d (abf2::URA3) strain grown in the presence of glucose loses its mitochondrial genome, whereas the KLR71 (abf2::URA3, pY-D3) strain maintains [rho⁺] mitochondria. Cells were grown to stationary phase on YPD (2% glucose), streaked on YPDGE (1% yeast extract, 2% peptone, 0.1% glucose, 3% glycerol, 2% ethanol) plates, and then incubated at 30°C. The cells from large colonies of each strain were picked and stained with a DNA-specific dye, DAPI, and visualized by fluorescence microscopy (middle) and phase-contrast microscopy (left). Magnification, ×400. The major fluorescence source in the central region of the cells corresponds to DAPI-stained nuclear DNA. Small and weak fluorescent spots corresponding to mtDNA in the periphery are observed only in cells of KLR71 (abf2::URA3, pY-D3).