FIG. 3.

Alignment and hydropathy plot of the deduced amino acid sequence of YHM2. (A) Multiple-sequence alignment for maximal amino acid similarities between Yhm2p and the members of the mitochondrial carrier family obtained with the ClustalW program in MacVector 6.01. The AAC2 gene encodes the mitochondrial ADP/ATP carrier (29), and ACR1 encodes a succinate/fumarate carrier (39). MRS3 encodes the suppressor of the mtRNA splicing defect (55, 56), and RIM2 encodes the suppressor of the pif1 mutant (50). YHM1 was isolated as a suppressor of the abf2 mutant (21, 34). The putative transmembrane (TM) domains are indicated, and the conserved motifs of mitochondrial energy transfer proteins are underlined. (B) Hydrophobicity profile of Yhm2p. The Yhm2p amino acid sequence was analyzed with the DNA Strider 1.2 program by the Kyte and Doolittle method. The most hydrophilic region (ILP3) of YHM2 is indicated.