TABLE 2.
Generation times of various strains with different carbon source at 30°C
| Strain | Generation time (h)a in:
|
|||||
|---|---|---|---|---|---|---|
| Rich mediumb
|
Synthetic mediumb
|
|||||
| Glucose | Glycerol | Glucose | Glycerol | Acetate | Ethanol | |
| MCY41 (yhm2::TRP1) | 2.2 | 3.4 | 3.4 | 7.5 | 6.4 | 11.8 |
| MCY42 (YHM2) | 2.3 | 3.6 | 3.2 | 4.3 | 5.1 | 5.7 |
| MCY41-1 (yhm2::TRP1/pY-D3) | 2.1 | 3.4 | 3.1 | 3.9 | 5.0 | 5.5 |
| MCY41-2 (yhm2::TRP1/pY-D3PRM) | 2.3 | 3.4 | 3.4 | 7.3 | 6.2 | 12 |
| KLR17-3d (abf2) | 3.3 | 7.1 | ||||
| KLR71 (abf2/pY-D3) | 2.5 | 4.2 | ||||
| KLR73 (abf2/pY-D3PRM) | 3.5 | NDc | ||||
a
Strains were diluted from overnight YPG cultures and grown in the presence of different carbon sources under aeration at 30°C. Growth was monitored by measuring the OD600 for four to five generations.
b
Carbon sources are given. See “Media and yeast strains” in Materials and Methods.
c
ND, not determined. The growth rates of KLR73 on nonfermentable carbon sources could not be determined because the strain grew very poorly.