HT-CETSA (Approaches) Table:
| HT-CETSA Approach | Detection | Size (aa) | Substrate/detection | Reporter Tagg | Heating method | Refs |
|---|---|---|---|---|---|---|
| NaLTSA | NanoLuc | 171 | Furimazine | 69.6 (naltsa); ~58 (purified) | PCR plates | 22 |
| SplitLuc-CETSA | SplitLuc | 15 | Furimazine | >90 (SOD1) | PCR plates (384w) or flat heat block (1536w) | 39 |
| HTDR-CETSA | ePL (B-gal complementation) | 42 | Chemiluminescent β-gal substrate | N/A | PCR plates | 35 |
| AlphaScreen | Antibody pair | N/A | N/A | N/A | PCR plates | 15–17 |
| High-content imaging | Antibody | N/A | Fluorescent secondary antibody | N/A | Water bath (submerged) or flat heat block | 19, 21 |