HT-CETSA (experimental details) Table:
| Target | Approach | Cell Line | Cell Density (x10^6 / mL) | Cell Number (x10^5) | Treatment dose | Treatment duration (h) | Heating duration (min) | Well format | Refs |
|---|---|---|---|---|---|---|---|---|---|
| B-Raf | AlphaScreen | A375 | 15 | 6.0 | 10 uM | 1–2 | 3 | 384 | 15 |
| PARP1 | AlphaScreen | MDA-MB-436 | 4 | 2.0 | 10 uM | 1–2 | 3 | 384 | 15 |
| TS | AlphaScreen | K562 | 10 | 1.0 | 50 uM | 2 | 3 | 384 | 17 |
| P38a | AlphaScreen | HL60 | not reported | 0.16 | DR | 30 | 3 | 384 | 16 |
| P38a | NaLTSA | HeLa | 0.20 | 0.20 | 10 uM | 0.5–1 | 3 | 96 | 22 |
| P38a | Imaging | A-431 | adherent | 0.02 | 50 uM | 0.5 | 3 | 384 | 21 |
| Chk1 | Imaging | HT29 U2OS | adherent | 10 | DR | 0.17 | 3 | 96 | 19 |
| SYMD3 | HTDR-CETSA β-gal EFC | HeLa | 0.40 | 0.05 | DR | 2 | 3 | 384 | 35 |
| Ido1 | HTDR-CETSA β-gal EFC | HeLa | 0.40 | 0.05 | DR | 2 | 3 | 384 | 35 |
| IDH1 | SplitLuc | HEK293 | 1.0 | 0.15 | DR | 1 | 3.5 | 384 | 39 |
| LDHA | SplitLuc | HEK293 | 1.0 | 0.05 | DR | 1 | 3.5 | 1536 | 39 |
| ALDH1A1 | SplitLuc | HEK293 | 1.0 | 0.05 | DR | 1 | 3.5 | 1536 | 39 |
| CDK9 | SplitLuc | HEK293 | 1.0 | 0.05 | DR | 1 | 3.5 | 1536 | 39 |