FIG. 3.

Different receptors preferentially interact with different domains of SMRT in a two-hybrid assay in vivo. pSG5 vectors expressing GAL4DBD (DBD) only or GAL4DBD fused with different domains of SMRT (as indicated below each panel) were introduced into CV-1 cells together with a GAL4 (17-mer)-luciferase reporter and a series of pSG5 GAL4AD constructs. The GAL4AD constructs contained GAL4AD only (open or cross-hatched bars) or a GAL4AD-receptor fusion (closed or hatched bars). The cells were incubated in the absence (open or closed bars) or presence (cross-hatched or hatched bars) of cognate hormone; after 48 h, the cells were harvested and luciferase activity was determined relative to that of pCH110, used as an internal control (Relative Luc). The results represent the averages and standard deviations from at least two duplicate experiments. (A) GAL4AD-T3Rα fusion. (B) GAL4AD-RARα fusion. (C) GAL4AD-RXRα fusion. (D) GAL4AD-VDR fusion.