FIG. 4.

Different receptors interact with different domains of N-CoR in a two-hybrid assay in vivo. pSG5 vectors expressing GAL4DBD only (DBD) or GAL4DBD fused with the domains of N-CoR indicated below the panels (corresponding to the schematic in Fig. 1B) were introduced into CV-1 cells together with a GAL4 (17-mer)-luciferase reporter and a series of pSG5 GAL4AD constructs. The GAL4AD constructs contained GAL4AD only (open or grey bars) or a GAL4AD-receptor fusion (black or hatched bars). The cells were incubated in the absence (open or closed bars) or presence (grey or hatched bars) of cognate hormone; after 48 h, the cells were harvested and luciferase activity was determined relative to that of pCH110, used as an internal control (Relative Luc). The results represent the averages and standard deviations from at least two duplicate experiments. (A) GAL4AD-T3Rα fusion. (B) GAL4AD-RARα fusion.