Fig. 3. (a) Representative multicolor fluorescent micrographs of A549 cells grown under normoxic (20% O2) or hypoxic (1% O2) conditions for 24 hours and then incubated with 10 μM of RNP for 1 hour, emission filter channels: DAPI (435–485 nm), Cy5 (663–737 nm), Cy7 (780–900 nm), length scale = 20 μm; ratiometric (Cy7/Cy5) image. (b) Quantification of average Mean Pixel Intensity (MPI) for multiple micrographs (N = 18 each condition). (c) Comparison of MPI ratio (Cy7/Cy5) for multiple micrographs of normoxic or hypoxic cells (N = 18 for each condition). **p ≤ 0.01, ***p ≤ 0.001.
