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. 2023 Oct 3;40(12):e15227. doi: 10.1111/dme.15227

FIGURE 2.

FIGURE 2

Mesenchymal stromal cell (MSC)‐derived cocktail pretreatment enhances insulin gene expression and content. Human islet extracts were generated immediately post‐isolation (day 1 for tissue bank) and after 72 h in culture (day 4) from untreated islets (control:Ctrl) or from islets treated with the MSC‐derived cocktail. (a, c) Preproinsulin mRNA (INS) and amylase mRNA (AMY1A) expression were quantified relative to housekeeping genes in islets from donor #5. Cocktail pretreatment enhanced the expression of INS mRNA (a) but had no effect on AMY1A mRNA (c), which was greatly reduced during 4 days in culture. These data were confirmed in measurements from four islets donors (#5–8) as shown in the Superplots in (b, d). Cocktail pretreatment also increased insulin protein content in the three islet preparations tested (donors #5–7), as shown in panel (e). Data were analysed by Student's unpaired t‐tests: *p < 0.05 cocktail pretreated versus untreated control islets. Same colour of two data points represents results from the same human donor as shown in Table 1.