FIG. 2.
Dose-response relationship of cAMP pulse-induced gene induction in wild-type cells. AX3 or aca cells were harvested in late log phase and stimulated in the presence (AX3 cells) or absence (aca cells) of 5 mM caffeine and the indicated concentrations of cAMP pulses delivered at 6-min intervals. RNA was isolated after 6 h of incubation and probed with 32P-labeled csA cDNA. The inset shows results of an experiment with aca cells, while the graph indicates means and standard deviations of results from two experiments with AX3 and caffeine and one experiment with aca cells.