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Phenotype of cAR mutants after cAMP treatment. After treatment with either 30 nM cAMP pulses (RI9, act15cAR1, and act15cAR3) or 600 nM cAMP pulses (act15cAR2) for 6 h and after subsequent incubation with 300 μM cAMP for 10 h, aliquots of 10 μl of 108 cells/ml were placed on nonnutrient agar and left to develop at 22°C for 24 h. Wild-type AX3 cells (WT) were placed directly on nonnutrient agar after being harvested from growth medium.
