FIG. 5.

Effects of adenosine analogues on prespore gene expression. ^act15cAR1 and ^act15cAR3 cells were prestimulated for 6 h with 30 nM cAMP pulses, and ^act15cAR2 cells were prestimulated with 600 nM cAMP pulses. Cells were incubated for an additional 8 h in DB in the absence and presence of 30 μM Sp-cAMPS and the indicated concentrations of adenosine, IPA, or NECA. RNA was isolated and probed with ³²P-labeled psA and CotC cDNAs. The experiment was repeated once with similar results.