Fig. 2.

Defective mitochondrial phenotype in chemoresistant cells. A Oxygen rate consumption (OCR) was determined in A2780 and A2780 CIS cells with Seahorse Mito stress kit assay. B OCR and Extracellular Acidification Rate (ECAR) obtained with Seahorse instruments allowed to determine the energetic map of A2780 and A2780 CIS cell lines. C-H Basal respiration, maximal respiration, ATP-production coupled respiration, proton leak, spare respiration capacity and non-mitochondrial oxygen consumption were determined by Seahorse data elaboration. I Glycolytic Proton Efflux Rate (GlycoPER) was determined with the Seahorse Glycolytic Rate Assay kit. J-L Basal Glycolysis, Basal PER, and Compensatory Glycolysis were determined by Seahorse data elaboration. M ATP production Rate was measured with Seahorse instrument and N-Q subsequent elaboration data have allowed determining ATP produced by mitochondria (MitoATP), by the glycolysis route (GlycoATP), total ATP and MitoATP/GlycoATP ratio. Data represent the mean ± SEM of at least three independent experiments. * p < 0.05; ** p < 0.01; ***p < 0.001