TABLE 1.

Anti HRV-2 activities of anilinoquinazoline-based kinase inhibitor hits^a

Compound	Structure	EC50 (µM)
AZ5385		0.5
AZ4514		4.4
AZ4516		3
AZ6252		1.5
AZ2538		1.5
AZ5756		1.5
AZ5765		1.5
AZ6610		1.5
AZ6636		1.5
AZ8146		4.4
AZ3426		5.8
AZ5719		4.4
Tyrphostin AG1478		>4b
Gefitinib		>4b

^a Specific hits, identified by screening of the kinase inhibitor library against HRV-2, were tested for anti-HRV-2 activity in a concentration-dependent inhibition of viral cytopathic effect assay in HeLa cells. Three separate determinations were performed, and the EC₅₀ values were calculated according to a Reed and Muench formula. Known EGFR-TKI AG1478 and gefitinib were also included for comparative purposes.

^b Lack of anti-HRV-2 activity at the highest non-cytotoxic concentration.