TABLE 3.

Mutations in HRV-2 variants selected by passaging in the presence of AZ5385

Protein	Alterationa in
	Control virus passage 11			AZ5385 virus passage 11
	V1b	V2	V3	V1	V2	V3
VP1				t2852a S181T	t2852a S181T	t2852a S181T
VP1				a2939g T210A	a2939gc T210A	a2939g T210A
Polymerase				t6610c silent	t6610c silent	t6610c silent

^a Mutations found in HRV-2 passaged in the absence (control virus passage) or the presence of inhibitor as related to original HRV-2. Nucleotide substitutions are denoted with lowercase letters and followed by predicted amino acid changes. Numbering of nucleotide is based on the GenBank HRV-A2 strain X02316. Numbering of amino acid residues is based on published cleavage sites of HRV-2 polyprotein (UniProt, P04936).

^b Viral variants (V1–V3) purified by limiting dilution method.

^c Substitution accompanied by a background of original base.