TABLE 2.
Mating efficiencies
| Relevant genotype
|
Mating efficiency (%)a | |
|---|---|---|
| MATa strain | MATα strain | |
| Wild type | Wild type | 90.3 ± 2.4 |
| ste11Δ | Wild type | <0.0001 |
| ste50Δ | Wild type | 34.7 ± 6.1 |
| ste50Δ | ste50Δ | 0.8 ± 0.2 |
Determined by mixing exponentially growing MATa cells with a fourfold excess of MATα cells (see Materials and Methods) and calculated as [(number of diploid cells formed)/(number of MATa cells)] × 100. Data represents means ± standard deviations of results from three independent experiments.