Table 2.
Specificity of the Lectins Used in the Study
| Lectin | Specificity for Sugar of N-Glycan Structures |
|---|---|
| MAA (Maackia amurensis agglutinin) | SA α2,3-linked35 |
| SNA (Sambucus nigra agglutinin) | SA α2,6-linked35 |
| RCA-I (Ricinus communis agglutinin I) | Terminal Gal36 |
| GSL-II (Griffonia simplicifolia lectin II) | GlcNAc, galactosylated tri-/tetra-antennary glycans35 |
| AAL (Aleuria aurantia lectin) | Fucose α1,6-linked to the core GlcNAc of N-glycans and with lower affinity to fucoses of the outer arms α1,2-, α1,3- and α1,4-linked37 |
| LCA (Lens culinaris agglutinin) | Recognize sequences containing fucosylated tri-mannose N-glycan core sites38 |
| LTA (Lotus tetragonolobus agglutinin) | Specifically react with fucose α1,3-linked to antennary GlcNAc39 |
| UEA (Ulex europaeus agglutinin) | Recognize antennary fucose α1,2-linked to Gal and α1,3-linked to GlcNAc40 |
| PHA-L (Phaseolus vulgaris lectin) | Recognize β1,6 branches of tri- and tetra-antennary oligosaccharides of N-glycans41 |
| Lectin | Specificity for sugar of O-glycan structures |
| MPL (Maclura pomifera lectin) | T (Galβ1,3GalNAc) and Tn antigen (single GalNAc)42 |
| VVL (Vicia villosa lectin) | Tn antigen (single GalNAc)43,44 |
| Jacalin (Artocarpus integrifolia lectin) | T antigen (Galβ1,3GalNAc), Tn antigen (single GalNAc), ST antigen (NeuAcα2,6Galβ1,3GalNAc)45 |
Abbreviations: SA, sialic acid; Gal, galactose; GalNAc, N-acetylgalactosamine; GlcNAc, N-acetylglucosamine; NeuAc, N-acetylneuraminic acid.