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. 2023 Dec 17;11(9):2303366. doi: 10.1002/advs.202303366

Figure 6.

Figure 6

Robust antibody response induced by mosaic in cynomolgus monkeys. A) Immunization and sample collection procedures for cynomolgus monkeys. B) SARS‐CoV, MERS‐CoV, and SARS‐CoV‐2 RBDs‐specific IgG titres at the indicated time points. Data shown are geometric means ± SEM. n = 3 monkeys in PBS/SAS group; n = 4 monkeys in mosaic RBD‐np/SAS group. Plasma was pooled by group. Three independent experiments were analyzed. Statistical analyses were performed using two‐way ANOVA. C) Neutralization titres against SARS‐CoV‐2 WT, SARS‐CoV‐2 VOCs, and MERS‐CoV EMC at the indicated time points using FRNT. Neutralization titres against MERS‐CoV GD01 and Nigeria, SARS‐CoV at the indicated time points using the pseudovirus assay. D) Four days after transduction with 2.5 × 108 PFU of Ad5‐hACE2, mouses were intravenous injected with 150 µl plasma from monkeys immunized with PBS/SAS, mosaic RBD‐np/SAS. 24 h later, mice were infected with 1 × 105 FFU of SARS‐CoV‐2 WT. E) Viral load in lung is expressed as FFU/g tissue. Statistical analyses were performed using two‐way ANOVA.