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PTB relocates to the cytoplasm early after PV infection and prior to cleavage by the PV 3Cpro protease. (A) HeLa R19 cells infected with PV1(S) were fixed at the indicated time points p.i. The subcellular localization of PTB or SC35 at the indicated time points was determined by indirect immunofluorescence; the nuclei were stained with 4′,6-diamidino-2-phenylindole. (B) The integrity of PTB was assayed by Western blot analysis of lysates collected at the indicated times p.i.
