FIG. 3.

Inhibition of transgene expression by silencing of HCV NS5B. Huh-NNRZ cells were infected with AdsiNS5B at an MOI of 80, 30, and 10 and then transfected with pT7cRLNS5B1 and pAM8-1. The transduced cells were harvested at day 2 posttransfection. HCV replicon RNAs were quantified with real-time RT-PCR (A), and relative Renilla luciferase activities were determined as described for Fig. 1 (B). The columns and bars represent the means and standard deviations of two independent experiments.