FIG. 1.

Coimmunoprecipitation of gH and VP16 from infected cells. Cell lysates were prepared from cells infected with HSV (wild type [WT]) or gH-null HSV (gH−) or from uninfected cells (U). Lysates were incubated (+) or not incubated (−) with mouse anti-VP16 monoclonal antibody at 37°C. Total cell lysates (CL: lanes 1 to 3), immunoprecipitated (bound) material (lanes 4 to 8), and unbound material (lanes 9 to 13) were subjected to SDS-PAGE, Western blotted, and probed with anti-gH and anti-VP16 antisera as indicated at the left. Positions and masses (in kilodaltons) of molecular mass markers are indicated by arrows. Note that 1/10 the total unbound material was run on the gels.