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Temperature dependence of VP16/gH coimmunoprecipitation. Coimmunoprecipitation experiments were performed as described in the legend to Fig. 1, except that incubation was done at 37°C, room temperature (RT), or 4°C. Western blotting was performed with total cell lysates (CL: lane 1) as well as bound (lanes 2 to 7) and unbound (lanes 8 to 13) material as described in the legend to Fig. 1. Note that 1/10 the total unbound material was run on the gels.
