FIGURE 5.

The interaction of FUS and TDP-43 is associated with RNA binding. (A) Cells transfected with FUS and TDP-43. FUS and TDP-43 have interaction (first panel). Cells transfected with FUS and TDP-43-2KQ. The protein interaction of FUS and TDP-43-2KQ decreases (second panel). Cells transfected with TDP-43 and FUS RNA-binding deficient mutant 2RA. The protein interaction of FUS-2RA and TDP-43 decreases (third panel). Cells were treated with 25 µM AdOx for 24 h to induce LLPS of FUS. (B) Protein interaction was compared by quantifying the ratio of red fluorescence in nucleolar and nuclear based on Figure 5A and Supplemental Figure S8. (C) Quantification of the ratio of red fluorescence in nucleolar and nuclear based on Figure 5B and Supplemental Figure S9. The t test was carried out for 50 cells, p < 0.0001. Scale bar = 10 µm.