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. 2024 Jan 29;35(3):ar34. doi: 10.1091/mbc.E23-09-0347

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© 2024 Bhatnagar et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 9: — Interaction between p62 and AMPs: HEK293T cells were transfected with pFLAG-Bst2 and pMY-IP-p62-HA (A), pCMV-HA-hIFITM3 and pMY-IP-p62-FLAG (B) and, pCMV-HA-hIFITM2 and pMY-IP-p62-FLAG (C),. Cell lysates were immunoprecipitated using anti-FLAG or anti-HA antibodies to pull down p62 and the precipitates were probed using anti-FLAG and anti-HA antibodies to detect interaction of AMPs. Changes in the levels of expression of IFITM2, IFITM3, Bst2 and p62 in cell lysate fractions of RAW GFP-LC3 cells with respect to the duration of LPS stimulation (D). Interaction of AMP with STM: RAW GFP-LC3 prestimulated with LPS for 24 h and infected with STM-mCherry and immunostained for endogenous levels of IFITM2, IFITM3 or, Bst2 at 12 hpi (E). Scale bars, 5 μm. Colocalization of GFP-LC3, AMP with phagosomal bacteria is marked by yellow arrows.