FIGURE 3:

Rab11a GTPase activity is necessary for the presence of Atg9A at dendritic spines. (A) Representative images of DIV22 neurons that were cotransfected with plasmids encoding GFP-Rab11a, DN Rab11a, or constitutively active (CA) Rab11a (green) together with Scarlet-I-Atg9A (magenta) and iRFP702 (blue). Imaging occurred 17–24 h after transfection. Scale bar = 5 µm. (B) Colocalization rate (percentage of colocalized vesicles) of transfected Rab11 variant and Atg9a at dendritic spines. (C) The number of Atg9a particles per spine of neurons transfected with the indicated Rab11 variants. Data are presented as the mean percentage of colocalized Rab11a particles normalized to all particles (B) or the number of Atg9A particles normalized to the number of spines for all cells (C). Number of analyzed cells: n = 30 for Rab11a WT, n = 30 for DN Rab11, and n = 27 for CA Rab11a from three independent experiments. ***p ≤ 0.001 (one-way ANOVA followed by Sidak’s post hoc test).