FIG. 7.

Glioblastoma-adapted VSV-rp30: attenuation of control cell infection. (A) Virus suspensions were tested with plaque assays prior to each experiment to ensure equal viral load for this comparative study. (B) VSV-rp30 was considerably attenuated for infection and cytotoxicity of normal human fibroblasts compared to the original VSV. (C) Coculture infection. Tumor cells were grown on a glass chip and transferred onto a fibroblast layer prior to adding either VSV-G/GFP or VSV-rp30 at an MOI of 1. The photomicrographs illustrate patterns of GFP expression and presence of cytopathic effects at 24 hpi (two left columns) and the long-term outcome at 7 dpi (two right columns).