FIG. 7.

Colocalization of CortBP1 and cortactin in 10T1/2 cells. 10T1/2 (a to d) and 10T1/2-c-Src (e to h) cells were transfected with the Myc-CortBP1cte expression vector and fixed 16 to 18 h posttransfection. Cells were then immunostained either with anti-CortBP1 in combination with the anti-cortactin MAb 4F11 (a, b, e, and f) or with 9E10 in combination with the cortactin-specific antibody anti-Cterm (c, d, g, and h). Arrowheads indicate lamellipodia and membrane ruffles in 10T1/2 cells and podosome-like structures in 10T1/2-c-Src cells. Bar, 20 μm.