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Site-directed mutagenesis underlines the importance of the EAV LTH structure for sg RNA synthesis. (A) Schematic representation of the mutations introduced into the LTH structure of the parental construct pD5DΔ1-163 (see the legend to Fig. 2). (B) Hybridization analysis of virus-specific RNA isolated at 15 h p.t. from BHK-21 cells transfected with D5DΔ1-163 RNA or RNAs from the mutants depicted in panel A.
