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A D5D derivative with two wt LTH domains, including two wt leader TRSs, produces a perfect double-nested set of sg mRNAs. A schematic representation of the wt genome and constructs D5DcmΔ1-163 and D5DcmΔ1-163\2xwt is shown on the left (see the legend to Fig. 2). On the right, a hybridization analysis of intracellular RNA at 15 h p.t. is shown.
