FIG. 3.

Nipah virus V and W proteins inhibit IRF3-responsive promoter activation by virus and intracellular dsRNA. (A and B) 293T cells were transfected with either the IFNβ-CAT (A) or ISG54-CAT (B) reporter plasmids and the indicated expression construct. Transfected cells were either mock infected or infected with 25 μl of SeV (10 hemagglutination units/ml). (C) 293T cells were first transfected with the ISG54-CAT reporter plasmid and the indicated expression plasmids and then, 1 day later, were either mock transfected or transfected with 20 μg of poly(I-C). CAT and luciferase activities were assayed 1 day postinfection or poly(I-C) transfection, and all data are expressed as the fold activation (compared to untreated control) of relative CAT activity as determined by normalization to a constitutively expressed Renilla luciferase control. Error bars indicate the means ± the standard deviations (SD) of two (A and B) or three (C) experiments.