FIG. 3.

Electrophoretic analysis of RNA extracted from FHV particles synthesized in the presence or absence of RNA1 replication. Sf21 cells were infected with the indicated baculoviruses and transfected 24 h later with in vitro-synthesized RNA2 (R2ts) in the presence (+) or absence (−) of RNA3 as shown (the molar ratio of RNA2 to RNA3 was approximately 1:3.6). All cells supported the replication of RNA2, but only cells infected with AcR1δ or AcR1δ[−5′UTR] supported the replication of RNA1. FHV particles were purified from the cells, their RNA extracted, and 1 μg run on a nondenaturing agarose gel. RNA isolated from authentic FHV particles (vRNA) was run for comparison in lane 5. The molecular sizes of RNA markers (in nucleotides) are indicated to the left.